Source: http://www.getoutdoors.com/goblog/index.php?/archives/4521-50%-Off-At-Backcountry.html
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Saturday, September 22, 2012
Kazakh special forces kill four militants in oil region
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Born to Die
A tragic love story over the course of two years between two different couples.
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Everyone should be loved for who they are not what they wear or what they look like, everyone deserves to be loved by someone!
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Friday, September 21, 2012
Reds' Baker misses another game with health issue
Cincinnati Reds manager Dusty Baker watches from the dugout in the seventh inning during a baseball game against the Miami Marlins, Friday, Sept. 14, 2012, in Miami. The Marlins defeated the Reds 4-0. (AP Photo/Lynne Sladky)
Cincinnati Reds manager Dusty Baker watches from the dugout in the seventh inning during a baseball game against the Miami Marlins, Friday, Sept. 14, 2012, in Miami. The Marlins defeated the Reds 4-0. (AP Photo/Lynne Sladky)
Cincinnati Reds manager Dusty Baker watches during the seventh inning of a baseball game against the Chicago Cubs in Chicago, Tuesday, Sept. 18, 2012. The game is Baker's 3,000th as a manager in the majors. (AP Photo/Paul Beaty)
Cincinnati Reds manager Dusty Baker sits in the dugout during the second inning of a baseball game between the Reds and the Chicago Cubs in Chicago, Tuesday, Sept. 18, 2012. The game is Baker's 3,000th as a manager in the majors. (AP Photo/Paul Beaty)
CINCINNATI (AP) ? Rookie shortstop Zack Cozart considered what it would be like to celebrate a division title without the manager who made it possible. It wouldn't be nearly so satisfying without Dusty Baker doing some dousing.
The Reds missed a third straight game on Friday because of problems caused by an irregular heartbeat. Doctors in Chicago advised him to spend one more night in a hospital there before traveling to Cincinnati for a series against the Los Angeles Dodgers.
The Reds' clinching number was down to one after the St. Louis Cardinals lost on Friday in Chicago 5-4 in 11 innings, setting up the possibility that Cincinnati could end up celebrating later in the night without its manager.
"It would be bittersweet," Cozart said. "We're all still thinking about him and wish he were here. He'll be with us in spirit. I guess we've got to go out there and win one for the skipper."
The 63-year-old Baker was hospitalized in Chicago on Wednesday because of symptoms from an irregular heartbeat that he's had for some time. The Reds swept the series against the Cubs, putting themselves in position to clinch their second division title in three years at home.
The Reds weren't sure when Baker would be allowed to rejoin the team.
"He's fine," said bench coach Chris Speier, who took over Baker's duties. "They want to make sure before they put him on a plane ? he has some fluids (built up) ? make sure that's all taken care of. But yeah, you worry about him. He's our leader, and for me, just a dear, dear friend. My mind is more with him than it is about this game."
The Reds clinched at least a wild-card berth in Chicago, but weren't going to celebrate until they got the division title, even if they had to do it without Baker.
"It would be a little disappointing," infielder Todd Frazier said. "Knowing Dusty, he would want us to get it done as quickly as possible. If he's not here, I know he'll be celebrating with us in spirit."
Baker made out the lineups for the two games he missed in Chicago. Speier took care of it on Friday, deciding to rest left fielder Ryan Ludwick one more day to let a sore groin heal. He also put Cozart back in the starting lineup for the first time in two weeks. Cozart missed 14 games with strained muscles in his left side.
Speier wasn't sure whether left-handed closer Aroldis Chapman would be cleared by the medical staff to play in the series. He hadn't appeared in a game since Sept. 10 because of a tired pitching shoulder.
"If he's available and there's an opportunity to use him, I'm not going to hesitate," Speier said. "I don't know what role I would put him in the first time back, but it would be good to get him back on the mound."
The Dodgers were fading from the wild-card race after losing 10 of 14. Atlanta and St. Louis were in position to get the two NL wild cards, with Milwaukee 2? games back and the Dodgers three games out.
"We're in a very advantageous position to allow our guys to maybe take an extra day of rest, make sure they don't reinjure something," Speier said. "It's not like the club we're playing. They're fighting every day to get to those playoff scenarios. We're in a pretty good position."
And the playoffs were right in front of them.
The Cardinals' game was shown on the videoboard during batting practice at Great American Ball Park, replacing the customary music. The Dodgers were taking batting practice when the Cubs won it, drawing cheers from the few thousand fans in the stands.
Dodgers catcher Tim Federowicz was in the batting cage and paused at the sound of the cheers. Other Dodgers looked up and smiled, too, happy that one of the teams they're chasing for the wild card had lost.
Reds second baseman Brandon Phillips tweeted about the Cubs' rally from the clubhouse. The Reds knew they were one win away from clinching, and that Baker wouldn't be there if they did so.
"He's been so instrumental in everything we do," outfielder Drew Stubbs said. "He's our leader, from putting the lineups out there to managing pitch-by-pitch."
___
Follow Joe Kay on Twitter: http://twitter.com/apjoekay
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Ultimate Safaris Invests in Technology for Rural Youth | Adventure ...
This project was a joint effort between the Tou Trust, a group of Swiss friends and the school itself. The school provided the center with all its modifications which now houses the computer center, along with arranging for the provision of electricity and internet to the area, whilst the Tou Trust provided all the computer equipment and installations. Mr Maujakisiujani Tjivikua, the principle of the school, said; ?we are excited by the new addition to the school and extremely grateful for the generous donation. We have close links to Ultimate Safaris, and this partnership again proves to us that they are a truly Namibian company with the objective to improve the lives of the people they work with. Ultimate Safaris is really fulfilling their social responsibility and is showing great leadership when it comes to this.?
It was decided by the school to name the computer center in honor of Denise Nieman, an American tour operator who recently lost her life in a vehicle accident while in Namibia.
Source: http://www.adventuretravelnews.com/ultimate-safaris-invests-in-technology-for-rural-youth
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Video: Romney tries to calm troubled waters
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Source: http://www.msnbc.msn.com/id/21134540/vp/49099797#49099797
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Thursday, September 20, 2012
Get2Go challenges region's students | Sir Edmund Hillary Outdoor ...
TOUGH ASSIGNMENT: Casey Allum and Nicole Robinson from Waiopehu College in Levin memorise a problem-solving code in the Get2Go Challenge. Schools from across the country participated in the challenge which involved a range of activities, including kayaking, mountain biking, and orienteering.
Kayaking, mountain biking, orienteering and problem-solving ? school teams squared off in the Get2Go Challenge in Palmerston North yesterday.
The four-hour multi-sport contest was staged at the Hokowhitu Lagoon, where year 9 and 10 students from St Peter?s College, Tararua College, Wanganui High School, Manawatu College, Waiopehu College, Horowhenua College, and Rangitikei College battled it out.
The Sir Edmund Hillary Trust Outdoor Pursuits Centre (OPC) is on a nationwide search for the most extreme and adventurous students with its annual challenge.
Get2Go is in its seventh year and is a junior version of the tough five day-long Sir Ed Hillary Challenge that is contested by years 11 to 13 students. There are 12 Get2Go regional challenges and yesterday was Manawatu/Whanganui?s turn.
The winning team of each region will go on to the five-day grand final at the OPC?s base on Great Barrier Island.
The result of the Manawatu/Whanganui contest was not available last night.
OPC events manager Darren Ashmore described the Get2Go challenge as a bit of ?Amazing Race and Survivor mixed into one?.
?It?s about balancing team skills and a bit of strategy,? he said.
Mr Ashmore said the objective of Get2Go was not just to find a winner, but to get kids participating in outdoor activity and maybe inspire a future Olympic athlete.
?These sports are recreational but they can be high-level Olympic sports . . . one of these kids might try the kayak today and take it further.?
Ruahine White Water Club president Greg Sawyer, whose club supplied the kayaks for the day, hoped the event would encourage kids to pursue his sport and make use of the beautiful outdoors.
?We want to encourage kids to try outdoor sports, whether it be kayak or something else, and enjoy what New Zealand has to offer,? he said.
Tararua College physical education teacher Shelley Arends said the Get2Go challenge was a great chance for students to try sports other than the traditional rugby, netball and basketball. (Source: Fairfax Media)
Source: http://www.opc.org.nz/2012/09/get2go-challenges-regions-students/
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Japan gets cold feet on total nuclear phase-out
TOKYO (AP) ? Japan's Cabinet on Wednesday stopped short of committing to phase out nuclear power by 2040, backtracking from an advisory panel's recommendations in the face of opposition from pro-nuclear businesses and groups.
The decision came the same day Japan launched a new regulatory body to replace the agency whose lack of independence from the nuclear industry was blamed for contributing to last year's disaster.
While not endorsing the energy policy document calling for the phase-out released last week, the Cabinet ministers did vaguely agree to pursue its goals. The advisory panel, acknowledging public aversion to nuclear power since the Fukushima accident, had called on Japan to phase it out within three decades through greater reliance on renewable energy, more conservation and sustainable use of fossil fuels.
The Cabinet said only that it would take the policy document "into consideration" and would seek public support for the goals, while continuously reviewing the process and also trying to gain understanding from the international community. But the public in this case includes not only the general public, which has come out strongly against nuclear power, but also the nuclear industry and other business interests, as well as communities near nuclear plants that rely on them economically.
National Policy Minister Motohisa Furukawa said the gist of Japan's energy policy remains to phase out nuclear power, though it would take time. Furukawa vowed to push for green energy and to seek to curb carbon dioxide emissions.
The Cabinet's ambiguous endorsement has added to criticism that the policy revision may just be intended to win votes in elections expected within the next few months.
But business leaders praised the Cabinet's perceived backpedalling.
"It seems that (the Cabinet) did not mention specific targets such as 2030s or zero percent, so I assume we could avert the problem for the time being," said Masahiro Yonekura, chairman of an influential business lobby Keidanren, told reporters. In his last-ditch protest Tuesday, Yonekura called the phase-out plan "totally unacceptable" and threatened to quit a government panel he is representing a business group.
Nuclear energy made up about a third of the country's electricity before the March 11, 2011, earthquake-tsunami caused reactor meltdowns at the Fukushima Dai-ichi plant, and Japan had planned to increase that to 50 percent. Now nuclear power is highly unpopular, and only two of the country's 50 functioning reactions are on line while the government addresses public concerns about safety.
The new regulatory agency inaugurated Wednesday was delayed months by demands from opposition lawmakers for more dependency as well as opposition to appointees' pro-nuclear background. The five-member Nuclear Regulation Authority is headed by nuclear physicist and Fukushima native Shunichi Tanaka.
Opposition lawmakers and activists have raise questions about his credentials because he had been executive of the Japan Atomic Energy Agency, which promotes development of nuclear energy.
Tanaka, 67, has helped decontaminate areas around the Fukushima plant contaminated with radiation. But he is unpopular among some residents who say he has downplayed the potential risk of low-dose radiation exposure.
The nominees for the four other committee members are a current JAEA official, a radiation expert, a seismologist and a former diplomat who participated in a parliamentary investigation into the Fukushima crisis.
Their appointments also triggered huge public protests because Prime Minister Yoshihiko Noda decided without going through required parliamentary approval to meet the committee's Sept. 26 launch deadline.
The new unit combines the former regulator Nuclear Industrial and Safety Agency, the Nuclear Safety Commission and several other nuclear-related government departments. The new entity is attached to the Environment Ministry, a move intended to distance the regulators from the influence of nuclear energy promoters. NISA was in the industry ministry, which also promotes nuclear energy.
Several investigations have said collusion between the regulators and the utility that ran Fukushima helped set off the worst nuclear disaster since Chernobyl.
The energy policy the Cabinet advisory panel proposed last Friday calls for greater reliance on renewable energy, more conservation and sustainable use of fossil fuels to achieve a nuclear-free society by 2040.
Such a reversal of Japan's decades-long advocacy of nuclear power is popular with the public, but faces strong resistance from powerful business interests and communities where nuclear plants are located are loath to give up their huge government subsidies.
To blunt outright opposition, the energy plan left many details undecided, and among the biggest are spent fuel processing and radioactive waste disposal. That allows a fuel recycling program at a plant in northern Japan's Rokkasho to continue. It also leaves unanswered how Japan will avoid accumulating stockpiles of spent plutonium in violation of its non-proliferation commitments.
The proposed phase-out of nuclear power by the 2030s was to be achieved mainly by retiring aging reactors and not replacing them. It calls for limiting each reactor to a 40-year lifespan and for building no more new reactors.
___
Follow Mari Yamaguchi on Twitter: http://www.twitter.com/mariyamaguchi
Source: http://news.yahoo.com/japan-gets-cold-feet-total-nuclear-phase-081857083--finance.html
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PFT: AEG?s NFL plans are expected to proceed
APIt was much easier to follow all of the developments in the Saints? bounty scandal during the offseason, when games weren?t, you know, being played.? It?s harder to find the time necessary to delve into the nooks and crannies of the case during the season, but I?ve finally managed to sit down and scrutinize the sworn statements submitted by former Saints assistant coach Mike Cerullo and former Saints defensive coordinator Gregg Williams.
For each document, I?ve taken notes from the perspective of a lawyer representing the four players who now face reissued suspensions:? Saints linebacker Jonathan Vilma, Saints defensive end Will Smith, Browns linebacker Scott Fujita, and free-agent defensive end Anthony Hargrove.
1.? At paragraph 2, Cerullo?s statement says that he worked for the Saints from April 2007 until April 2010.? He doesn?t address why he left the Saints in April 2010.? So why did he leave?? Was he fired or did he quit?? Is there anything in his personnel file that would suggest issues with honesty?? Did he leave with an axe to grind?
2.? At paragraph 2, Cerullo says he worked as an offensive assistant from 2007 to 2008, and that he became a defensive quality control coach in 2009.? Why did he switch from one side of the ball to the other?? If it wasn?t working out on offense, why didn?t the Saints just let him go?
3.? At paragraph 3, Cerullo says he?s ?aware of and [has] provided additional information about the program to NFL investigators.?? What other information is he aware of and what else has he provided?
4.? At paragraph 3, Cerullo says he was assigned the task of administering the pay-for-performance program.? Did he have any qualms about the program?? Did he raise with Gregg Williams or anyone else the question of whether it complies with NFL rules?? Did he report to his former bosses on offense what was happening?
5.? At paragraph 5, Cerullo says dues of $100 were assessed on all participants in the program, before each game, along with fines for mental errors, lack of hustle, and a missed opportunity for a big play.? Who gave money, and how much did each person give?? Why haven?t they all been disciplined?
6.? At paragraph 9, Cerullo says that he was responsible for physically handing out the cash to players.? Who received cash, and how much did they receive?? Why haven?t they all been disciplined?
7.? At paragraph 10, Cerullo says his duties included keeping track of dues and fees owed, and that he made slides reminding players of overdue game dues and fines.? Again, who paid money into the program, and why haven?t they all been disciplined?
8.? At paragraph 11, Cerullo says that he was the ?lower court? for disputes about fines and payouts.? Who made complaints about money paid in and money received?
9.? At paragraph 13, Cerullo says that Vilma produced ?two five stacks? (i.e., $10,000) for anyone who knocked Kurt Warner out of the divisional playoff game, and that Cerullo collected the money and gave it to Williams for safekeeping.? Where did the money come from?? Was it Vilma?s own money, or did it come from someone else?? Was it money from the pay-for-performance pool?? Did Vilma engage in the exercise on his own or at the urging of someone else, like Williams?
10.? At paragraph 14, Cerullo says that the money wasn?t paid because Warner wasn?t knocked out of the game.? But he was, returning to the game later.? So was any of the money (or any other money) paid out as a ?cart-off? to Bobby McCray, who applied the post-interception hit to Warner?? If not, why not?
11.? At paragraph 15, Cerullo says that Fujita and Smith pledged money to the general pool in the meeting before the 2009 NFC title game, and that Cerullo was keeping track of the pledges.? Why does Cerullo?s statement not mention his handwritten notes indicating that assistant head coach Joe Vitt pledged $5,000?? Why did the NFL not discipline Vitt for this, or even investigate it?? Why weren?t the notes attached as an exhibit to the sworn statement?
12.? At paragraph 16, Cerullo says he involved in communications aimed at concealing the program from the league.? He says he was told to delete documents regarding the pay-for-performance program from his computer, and that he was present for a meeting between Vitt and Hargrove, during which Hargrove was told to deny any knowledge of the pay-for-performance program, and Hargrove said, ?I can lie with the best of them.?? If the allegations in Vilma?s lawsuit regarding Cerullo are accurate, why did the Saints include him in these communications?? Why didn?t the Saints negotiate a severance package that allowed him to talk about his employment with the Saints only if subject to a court order?
That last part, while irrelevant to the facts of the case, is perhaps the most amazing.? Regardless of why Cerullo spoke to the league and whether what he said is accurate, it?s common for employers to offer extra money to employees who may be disgruntled and/or who may know to much both to avoid a lawsuit and to buy their silence going forward.
Over the years, it?s become obvious that the Saints have a problem with disgruntled employees who are motivated to take action against the team.? The Saints also have a problem, in our view, in the legal department.? The Saints never should have let Cerullo leave without ensuring that he wouldn?t be able to blab later, and the lesson for the Saints and every other NFL team (and every other employer) is to be sure that procedures are in place to spot those employees who know enough to cause trouble later ? and to ensure that they legally can share that knowledge only if legally required to do so.
In this case, if Cerullo had received, say, an extra $10,000 in exchange for his silence, none of this ever would have come to light.
None of this makes the pay-for-performance program right or wrong.? It?s just a basic reality of managing risk and limiting potential liabilities.? In this case, someone should have realized that Cerullo could be a problem later.? In the movies, Cerullo would have been whacked.? In the real world, Cerullo would have gotten some extra money in his final paycheck and he would have signed a 10-page document that included language preventing him from blowing whistles or otherwise talking to anyone about anything unless and until he was legally required to do so.
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Brenda Council, Nebraska State Senator, Pleads Guilty To Filing False Campaign Finance Reports
FILE - In this May 19, 2009, file photo Omaha state Sen. Brenda Council of Omaha speaks in Lincoln, Neb. (AP Photo/Nati Harnik, File)
OMAHA, Neb. -- Nebraska state Sen. Brenda Council has pleaded guilty to filing false campaign finance reports that prosecutors say concealed her misuse of more than $60,000 in campaign donations at casinos.
A judge sentenced Council on Tuesday to pay a $500 fine and court costs.
She has apologized for making an error in judgment, and plans to continue running for re-election in northeast Omaha.
She says she's getting treatment for a gambling addiction.
Nebraska Attorney General Jon Bruning says bank records and casino security videos show that Council withdrew a total of $63,052 at casinos on several occasions and then deposited $36,166 in cash back into the campaign fund. Council has agreed to repay the $27,000 shortfall.
Council is running against Ernie Chambers, who was the longest-serving member of the Nebraska Legislature.
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Tuesday, September 18, 2012
Pa. high court wants review of voter ID access
HARRISBURG, Pa. (AP) ? Pennsylvania's highest court on Tuesday told a lower court that it should stop a tough new voter photo identification law from taking effect in this year's presidential election if the judge concludes voters cannot easily get ID cards or thinks they will be disenfranchised.
The 4-2 decision by the state Supreme Court sends the case back to the lower Commonwealth Court, where a judge initially ruled in August that the divisive law could go forward. The high court asked for an opinion by Oct. 2 ? just 35 days before the election.
If the judge finds there will be no voter disenfranchisement and that IDs are easily obtained, then the 6-month-old law can stand, the Supreme Court said.
But the Supreme Court's directions to the lower court set a much tougher standard than the one Judge Robert Simpson used when he rejected the plaintiffs' request to halt the law, said David Gersch, the challengers' lead lawyer.
"It's certainly a very positive step in the right direction in that the court recognizes that the state does not make adequate provision for people to get the ID that they would need to vote," Gersch said. "In addition, there is a practical problem with getting the ID to people in the short time available."
A spokesman for the Pennsylvania Department of State, which oversees voting and elections, said the agency will provide whatever information a judge may seek.
"We believe, as we have all along, that any legal voter who wants to get an ID is able to do so," spokesman Ron Ruman said.
A Commonwealth Court official said Tuesday afternoon that no judge had been assigned yet to the task.
The Republican-penned ID law passed over the objections of Democrats and ignited a furious debate over voting rights, making it a high-profile issue in the contest for the state's prized 20 electoral votes between President Barack Obama, a Democrat, and Republican nominee Mitt Romney, the former Massachusetts governor.
The court's three sitting Republican justices were joined in the majority by one of the court's Democrats, Max Baer. The court's two other Democrats dissented, saying enough evidence of voter disenfranchisement already exists to stop the law now.
The problem, the four majority justices noted, is that the state has had to scramble to solve impediments to distributing a secure, non-driver photo ID card promised under the law to any registered voter who needs one.
The justices noted that the state is unable to comply with that crucial provision because those cards are subject to federal rules requiring applicants to provide supplemental identification, such as an official record of birth. Some registered voters might not be able to produce that sort of identification.
In an effort to address that problem, the state began issuing new, voting-only ID cards in late August, after Simpson's initial ruling, that aren't subject to the stringent federal rules.
"Thus, we will return the matter to the Commonwealth Court to make a present assessment of the actual availability of the alternate identification cards on a developed record in light of the experience since the time the cards became available," the justices wrote.
Plaintiffs' lawyers, however, say registered voters are still having trouble getting the new voting-only cards. In some cases, state employees at driver license centers are having trouble verifying voter registration records, they said.
The state's Republican Party chairman, Rob Gleason, said Tuesday that he believes the lower court "will find that this law does not ? and will not ? disenfranchise voters in the state."
Republicans, long suspicious of ballot-box stuffing in the Democratic bastion of Philadelphia, maintain that the law will deter election fraud. But Democrats point to a blank trail of evidence of such fraud, and charge that Republicans are trying to steal the White House by making it harder for the elderly, disabled, minorities, the poor and college students to vote.
The law ? among the nation's toughest ? has inspired protests, warnings of Election Day chaos and voter education drives. It was already a political lightning rod when a top state Republican lawmaker boasted to a GOP dinner in June that the ID requirement "is going to allow Gov. Romney to win the state of Pennsylvania."
The plaintiffs ? eight registered Democrats, plus the Homeless Advocacy Project, the League of Women Voters of Pennsylvania and the Pennsylvania chapter of the National Association for the Advancement of Colored People ? had sought to block the law from taking effect in this year's election as part of a wider challenge to its constitutionality.
Republican Gov. Tom Corbett signed the law in March after every single Democratic lawmaker voted against it.
The prior law required identification only for people voting in a polling place for the first time and it allowed non-photo documents such as utility bills or bank statements.
Source: http://news.yahoo.com/pa-high-court-wants-review-voter-id-access-174935794.html
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Precision motion tracking -- thousands of cells at a time
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Public release date: 18-Sep-2012[ | E-mail |
Share ] Contact: Joshua A. Chamot
jchamot@nsf.gov
703-292-7730
National Science Foundation
Novel microscopy technique could open new windows into protozoan behavior, microbial diseases and fertility
Researchers have developed a new way to observe and track large numbers of rapidly moving objects under a microscope, capturing precise motion paths in three dimensions.
Over the course of the study--reported online Sept. 17, 2012, in the Proceedings of the National Academy of Sciences--researchers followed an unprecedented 24,000 rapidly moving cells over wide fields of view and through large sample volumes, recording each cell's path for as long as 20 seconds.
"We can very precisely track the motion of small things, more than a thousand of them at the same time, in parallel," says research lead and National Science Foundation CAREER awardee Aydogan Ozcan, an electrical engineering and bioengineering professor at UCLA. "We were able to achieve sub-micron accuracy over a large volume, allowing us to understand, statistically, how thousands of objects move in different ways."
The latest study is an extension of several years of NSF-supported work by Ozcan and his colleagues to develop lens-free, holographic microscopy techniques with applications for field-based detection of blood-borne diseases and other areas of tele-medicine. Those efforts recently resulted in a Popular Mechanics Breakthrough Award and a National Geographic Emerging Explorer Award, among others. Ozcan's research is also supported through an NIH Director's New Innovator Award, Office of Naval Research Young Investigator Award and an Army Research Office Young Investigator Award from the Department of Defense.
For the recent work, Ozcan and his colleagues--Ting-Wei Su, also of UCLA, and Liang Xue, of both UCLA and Nanjing University of Science and Technology in China--used offset beams of red and blue light to create holographic information that, when processed using sophisticated software, accurately reveal the paths of objects moving under a microscope. The researchers tracked several cohorts of more than 1,500 human male gamete cells over a relatively wide field of view (more than 17 square millimeters) and large sample volume (up to 17 cubic millimeters) over several seconds.
The technique, along with a novel software algorithm that the team developed to process observational data, revealed previously unknown statistical pathways for the cells. The researchers found that human male gamete cells travel in a series of twists and turns along a constantly changing path that occasionally follows a tight helix--a spiral that, 90 percent of the time, is in a clockwise (right-handed) direction.
Because only four to five percent of the cells in a given sample traveled in a helical path at any given time, researchers would not have been able to observe the rare behavior without the new high-throughput microscopy technique.
"This latest study is an extension of truly novel and creative work," says Leon Esterowitz, the NSF biophotonics program officer who has supported Ozcan's efforts. "The holographic technique could accelerate drug discovery and prove valuable for monitoring pharmaceutical treatments of dangerous microbial diseases."
The PNAS paper reports observations of 24,000 cells over the duration of the experiments. Such a large number of observations provide a statistically significant dataset and a useful methodology for potentially studying a range of subjects, from the impact of pharmaceuticals and other substances on large numbers of cells--in real time--to fertility treatments and drug development.
The same approach may also enable scientists to study quick-moving, single-celled microorganisms. Many of the dangerous protozoa found in unsanitary drinking water and rural bodies of water have only been observed in small samples moving through an area that is roughly two dimensional. The new lens-free holographic imaging technique could potentially reveal unknown elements of protozoan behavior and allow real-time testing of novel drug treatments to combat some of the most deadly forms of those microbes.
###
[ | E-mail | Share ] ?
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Public release date: 18-Sep-2012[ | E-mail |
Share ] Contact: Joshua A. Chamot
jchamot@nsf.gov
703-292-7730
National Science Foundation
Novel microscopy technique could open new windows into protozoan behavior, microbial diseases and fertility
Researchers have developed a new way to observe and track large numbers of rapidly moving objects under a microscope, capturing precise motion paths in three dimensions.
Over the course of the study--reported online Sept. 17, 2012, in the Proceedings of the National Academy of Sciences--researchers followed an unprecedented 24,000 rapidly moving cells over wide fields of view and through large sample volumes, recording each cell's path for as long as 20 seconds.
"We can very precisely track the motion of small things, more than a thousand of them at the same time, in parallel," says research lead and National Science Foundation CAREER awardee Aydogan Ozcan, an electrical engineering and bioengineering professor at UCLA. "We were able to achieve sub-micron accuracy over a large volume, allowing us to understand, statistically, how thousands of objects move in different ways."
The latest study is an extension of several years of NSF-supported work by Ozcan and his colleagues to develop lens-free, holographic microscopy techniques with applications for field-based detection of blood-borne diseases and other areas of tele-medicine. Those efforts recently resulted in a Popular Mechanics Breakthrough Award and a National Geographic Emerging Explorer Award, among others. Ozcan's research is also supported through an NIH Director's New Innovator Award, Office of Naval Research Young Investigator Award and an Army Research Office Young Investigator Award from the Department of Defense.
For the recent work, Ozcan and his colleagues--Ting-Wei Su, also of UCLA, and Liang Xue, of both UCLA and Nanjing University of Science and Technology in China--used offset beams of red and blue light to create holographic information that, when processed using sophisticated software, accurately reveal the paths of objects moving under a microscope. The researchers tracked several cohorts of more than 1,500 human male gamete cells over a relatively wide field of view (more than 17 square millimeters) and large sample volume (up to 17 cubic millimeters) over several seconds.
The technique, along with a novel software algorithm that the team developed to process observational data, revealed previously unknown statistical pathways for the cells. The researchers found that human male gamete cells travel in a series of twists and turns along a constantly changing path that occasionally follows a tight helix--a spiral that, 90 percent of the time, is in a clockwise (right-handed) direction.
Because only four to five percent of the cells in a given sample traveled in a helical path at any given time, researchers would not have been able to observe the rare behavior without the new high-throughput microscopy technique.
"This latest study is an extension of truly novel and creative work," says Leon Esterowitz, the NSF biophotonics program officer who has supported Ozcan's efforts. "The holographic technique could accelerate drug discovery and prove valuable for monitoring pharmaceutical treatments of dangerous microbial diseases."
The PNAS paper reports observations of 24,000 cells over the duration of the experiments. Such a large number of observations provide a statistically significant dataset and a useful methodology for potentially studying a range of subjects, from the impact of pharmaceuticals and other substances on large numbers of cells--in real time--to fertility treatments and drug development.
The same approach may also enable scientists to study quick-moving, single-celled microorganisms. Many of the dangerous protozoa found in unsanitary drinking water and rural bodies of water have only been observed in small samples moving through an area that is roughly two dimensional. The new lens-free holographic imaging technique could potentially reveal unknown elements of protozoan behavior and allow real-time testing of novel drug treatments to combat some of the most deadly forms of those microbes.
###
[ | E-mail | Share ] ?
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Source: http://www.eurekalert.org/pub_releases/2012-09/nsf-pmt091812.php
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